The Kinesin-4 Family

The Kinesin-4 (formerly Chromokinesin/KIF4) family tree was obtained using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-4 proteins from humans and several model organisms. Kinesin-4 proteins from other organisms are also shown in the table and sequence alignment below.

Species/protein	Molecular mass (kDa)	Motor polarity & velocity	Subcellular localization	Comments
D. melanogaster Klp3A		ND
H. sapines KIF4	140	ND
D. melanogaster Klp31E		ND
H. sapiens NYREN62	70
C. elegans T01G1		ND
A. thaliana MCA23.16	117
A. thaliana F11C1.80	121
A. thaliana MSL3.5	145
C. elegans Y43F4B		ND
D. melanogaster Klp31D		ND
G. gallus Chrkin	139	ND	Chromosome arms (chromokinesin)	Binds DNA
M. musculus KIF4	140	Plus, 2-12 µm/min	Membranous vesicles, mitotic spindle	Motor for vesicle transport?
X. laevis Klp1	139	ND	Chromosome arms	May position mitotic chromosomes, stabilize bipolar mitotic spindle

          1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360      
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DmKlp3A   DPSCVAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKDLFETCVQAKVKKLLNGYNVTILAYGQTGSGKTYTMGTAFN--GVLDDHV-GVIPRAVHDIFTAIAEMQ-SEF-----------RFAVTCSFVELYQEQFYDLFSSKT-RDKATVDIREVKNRIIMPGLTELVVTSAQQVTDHLIRGSAGRAVAATAMNETSSRSHAIFTLTLVATK-------------LDGKQSVTTSRFNLVDLAGSERCSKTLASGDRFKEGVNINKGLLALGNVINALGSG-QAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVAETLSTLRYADRALQIKNKPVVN  Dmklp3A 
HsKIF4    KGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGT-DKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTV-GVIPRVIQLLFKEIDKKSDFEF-------------TLKVSYLEIYNEEILDLLCPSR-EKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAILTISLEQGK-------------KSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDD-KKGGFAPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVN  HsKIF4
DmKlp31E  KDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGS-DKAFTFDYVFDTNSNQCDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFN-KNSNFKIHEDASGQITISGASIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQR-----LLTPSDNVPDNDLETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKIN  DmKlp31E
CeT01G1   NDRDLISVKSIRPQGNREKLEGSRVCTSVLPNDPQVTIGG-DRSFTYDHVFDMPTLQYVVYESCVEKLVDGLFDGYNATVLAYGQTGSGKTHTMGTAFDAAVTQKEED-LGVIPRAIQHTFRKIAECKAQA---IEQGLLEPAFEVSVQFVELYNDDVLDLLSDDRSMSSSIRIHEDSRGEIVLHGVEQRSVFDMHGTMDILKNGALNRTVAATNMNEQSSRSHAIFTLHLKQQRVAAPLDESGEQKTGELEMEMLCAKFHFVDLAGSERMKRTGATGDRAKEGISINVGLLALGNVIAALGGANGKVSHVPYRDSKLTRLLQDSLGGNSRTLMIACCSPMDSDFVETLNTMKYANRAKEIKNKVVAN  CeT01G1
CeY43F4B  SDASLRVVVRARPMNGRETKEGASRCVQFYENTKQIVINE-SATFTFDAVFADTSDQESVYETTALPLLDRIFAGFNATVLAYGQTGSGKTYTMGTEDN--VGTDEMRRGIIPRLVSALFQRIMNTE-APE-----------SFAVTVSMFEVYGDNVYDLLRPDK----VKLNVHGDEKNCTVVNLTAVPVIDLKGALKQLAVGCHYRTKAETAMNAMSSRSHAVFTVFVEKTA-------------TAECDSAFSAKLQLVDLAGSERLKKTEAEGNRMKEGININGGLLILSQVIAALAT---KQKHIPYRNSVITRVLQDSLGGNSFTVFLACISPADSNSQETLNTLRYADRAKQIKNKPIVN  CeY43F4B 
DmKlp31D  KDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGS-DKAFTFDYVFDTNSNQCDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFN-KNSNFKIHEDASGQITISGASIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQR-----LLTPSDNVPDNDLETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKIN  DmKlp31D
Ggchrkin  EKGIPVRVVRCRPLVPKETSEGCQMCLSFVPGEPQVIVGS-DKAFTYDYVFDPSVEQEEVFNTAVAPLIRGIFKGYNATVLAYGQTGSGKTYSMGGTYTASQEHDPSM-GVIPRVIKLLFKEKEQRQDWEF-------------VLKVSYLEIYNEDILDLLCSSRERSSQISIREDPKEGIKIVGLTERNVASARDTVSCLEQGNNCRTVASTAMNSQSSRSHAIFTICIDQKK-------------KNDKNSSFHSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGEENKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVN  Ggchrkin
MmKIF4    KGIPVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGN-DKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAI-GVIPRVIQLLFKEINKKSDFEF-------------TLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGLTEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRK-------------KNDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGDD-KKGNFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIIN  MmKIF4
Xlklp1    EGIPVRVALRCRPLVPKENNEGCKMCLTFVPGEQQVIVGT-EKSFTYDYVFDPSAEQEEVYNSAVAPLIKGLFKGYNATVLAYGQTGSGKTYSMGGAYTHNQENEPTV-GVIPRTVIALFREIHQRPEWEF-------------NLKVSYLEIYNEEILDLLYAARDKTNTISIREDPKEGIKICGLTERDVKTALDTLSCLEQGNSSRTVASTAMNSQSSRSHAIFTISIEQRK-------------EGDKNNSFRSKLHLVDLAGSERQKKTKAEGDRLKEGISINRGLLCLGNVISALGDESKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNMEETLNTLRYADRARKIKNKPIVN  Xlklp1