The Kinesin-13 Family

The Kinesin-13 (formerly MCAK/KIF2) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap values adjacent to the nodes are the percentages of 1,810 heuristic bootstrap trials in which the indicated groups were found. The tree contains Kinesin-13 proteins from humans and several model organisms. Kinesin-13 proteins from other organisms are also shown in the table and sequence alignment below. There are three Kinesin-13 proteins in Drosophila, the mouse and humans. Pairwise comparisons of full-length protein and motor domain sequences identify putative orthologues as [DmKlp10A, MmKif2A, HsKin2 (HsKif2)]; [DmKlp59C, MmKif2C, HsMCAK]; and [DmKlp59D, MmKif2B, HsKlp17] (C.D. Yankel & S.A. Endow, 2006). The Kinesin-13 proteins function in MT disassembly in mitosis and interphase.

Species/protein	Molecular mass (kDa)	Motor polarity & velocity	Subcellular localization	Comments
C. fusiformis DSK1	63	ND	Spindle
P. falciparum L2165w
D. melanogaster Klp10A		ND	ND
D. melanogaster Klp59D
D. melanogaster Klp59C		ND	ND
H. sapiens Klp17q22	76
H. sapiens Kin2	77	ND	ND
H. sapiens MCAK	81	ND	ND
C. elegans K11D9.c	77	ND	ND
A. thaliana MGL6.9	89
A. thaliana MSL1.9	77
L. major Kin	74	ND	ND
C. griseus MCAK	83	ND	Kinetochore	Localizes to kinetochore, anchors kinetochore MTs?
M. musculus KIF2	81	Plus, 28 µm/min	Membranous vesicles	Abundant in developing axons, transport of specific organlles?
M. musculus KIF2C	75	ND	ND
R. norvegicus KRP2	ND	ND	ND
X. laevis KCM1	83	ND	ND
X. laevis KIF2	ND	ND	ND

          1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340                
          '        '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '                  
CfDSK1    TNSNICIAVRKRPISDKERQKLDHDSVSCFQNKVWIHSAKLKVDGITKYLTHNSFQLDHTFGEDSTTEQIYLATTLPLVDHVVSTQGRATVFCYGQTGSGKTYTMNG---------IQQILAYDLYGQ-LAEHTDD-----LEITVAFFELYSGNVLDLLHGCQ-RCKLLEDGNGEVNITGLREVPAPTPEAFLQVIEEGHSLRTTQKTEANDASSRSHAICQVFLRDY-GGNLRGKLGLVDLAGSERGSDTKQHNSQRRTESADINTSLLALKECIRALGQKSAHVPYRGSKLTLILKDCF-SPDSKTTMVATVSPGASAADHSLNTLRYADRIKEQRVSSNGQ  CfDSK1 
DmKlp10A  DDHQITVCVRKRPISRKEVNRKEIDVISVPRKDMLIVHEPRSKVD-LTKFLENHKFRFDYAFNDTCDNAMVYKYTAKPLVKTIFEGGMATCFAYGQTGSGKTHTMGGEFNGKVQDCKNGIYAMAAKDVFVTLNMPRYRAMNLVVSASFFEIYSGKVFDLLSDKQ-KLRVLEDGKQQVQVVGLTEKVVDGVEEVLKLIQHGNAARTSGQTSANSNSSRSHAVFQIVLRPQGSTKIHGKFSFIDLAGNERGVDTSSADRQTRMEGAEINKSLLALKECIRALGKQSAHLPFRVSKLTQVLRDSFIGEKSKTCMIAMISPGLSSCEHTLNTLRYADRVKELVVKDIVE  DmKlp10A
DmKlp59C  NCHQIMVCVRKRPLRRKELADREQDVVSIPSKHTLVVHEPRKHVN-LVKFLENHSFRFDYVFDEECSNATVYEFTARPLIKHIFDGGMATCFAYGQTGSGKTYTMGGQFPGRHQSSMDGIYAMAAKDVFSTLKTVPYNKLNLKVYCSFFEIYGTRVFDLLMPGKPQLRVLEDRNQQVQVVGLTQNPVQNTAEVLDLLELGNSVRTSGHTSANSKSSRSHAVFQIVLRSAAGEKLHGKFSLIDLAGNERGADNSSADRQTRLEGSEINKSLLVLKECIRALGRQSSHLPFRGSKLTQVLRDSFIGKKVKTCMIAMISPCLHSVEHTLNTLRYADRVKELSVESIPS  DmKlp59C
HsKin2    DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTAKPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTAA  HsKin2
HsMCAK    EEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLNKKA-KLRVLEDGKQQVQVVGLQEHLVNSADDVIKMLDMGSACRTSGQTFANSNSSRSHACFQIILRAK--GRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPS  HsMCAK
CeK11D9.c NENRISVCVRKRPLNKKELTKAEVDVITIPSRDITILHQPQTRVD-LTKYLDNQKFRFDYSFDEYANNELVYRFTAAPLVKTVFDNGTATCFAYGQTGSGKTHTMGGDFSGKKQNASMGIYALTARDVFRMLEQPQYRRKDLSVHCAFFEIYGTKTYDLLNDKA-ELRVLEDKMQKVQVVGLKEEQAHNEQDVLELINKGTLVRTAGTTSANANSSRSHAIFQIILRQG--KKVWGKFSLIDLAGNERGQDTRECDRDTRKEGANINTSLLALKECIRGMARNSSHVPFRQSKLTMVLRDSFIGEKSRTVMISMISPGISSSDHTLNTLRYADRVKEMGTDGSGE  CeK11D9.c 
LmKin     RKSRILVAIRKRPLSAGEQTNGFTDIMDADNSGEIVLKEPKVKVD-LRKYTHVHRFFFDEVFDEACDNVDVYNRAARALIDTVFDGGCATCFAYGQTGSGKTHTMLGKGPEP------GLYALAAKDMFDRLTSD------TRIVVSFYEIYSGKLFDLLNGRR-PLRALEDDKGRVNIRGLTEHCSTSVEDLMTIIDQGSGVRSCGSTGANDTSSRSHAILEIKLKAKRTSKQSGKFTFIDLAGSERGADTVDCARQTRLEGAEINKSLLALKECIRFLDQNRKHVPFRGSKLTEVLRDSFIG-NCRTVMIGAVSPSNNNAEHTLNTLRYADRVKELKRNATER  LmKin
CgMCAK    EEHRICVCVRKRPLNKQELAKKEIDVISVPSKCLLFVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKSQNTSKGIYAMASRDVFLLKSQPRYRNLNLEVYVTFFEIYNGKVFDLLNKKA-KLRVLEDSKQQVQVVGLQEYLVNCADDVIKMLNMGSACRTSGQTFANSNSSRSHACFQILLRAK--GRLHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCEYTLNTLRYADRVKELSPHSGLS  CgMCAK
MmKIF2    DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLRLKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKEFGISPSDI  MmKIF2
MmKIF2C   DEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVD-LTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVNPAAA  MmKIF2C
RnKRP2    EEHRICVCVRKRPLNKQELAKKEIDVISVPSKCLLLVHEPKLKVD-LTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKSQNASKGIYAMASRDVFLLKNQPRYRSLNLEVYVTFFEIYNGKVFELLNKKA-KLRVLEDSKQQVQVVGLQEYLVTCADDVIKMINMGSACRTSGQTFANSNSSRSHACFQILLRAK--GRLHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKESIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCEYTLNTLRYADRVKELSPHSGPS  RnKRP2
XlKCM1    EEHRICVCVRKRPLNKQELSKKEIDIISVPSKNIVLVHEPKLKVD-LTKYLENQAFRFDFSFDETATNEVVYRFTARPLVQSIFEGGKATCFAYGQTGSGKTHTMGGDFSGKSQNVSKGVYAFASRDVFLLLDQPRYKHLDLDVFVTFFEIYNGKVFDLLNKKT-KLRVLEDAKQEVQVVGLLEKQVISADDVFKMIEIGSACRTSGQTFANTSSSRSHACLQIILRRG--SKLHGKFSLVDLAGNERGVDTASADRITRMKGAEINRSLLALKECIRALGQNKSHTPFRESKLTQILRDSFIGENSRTCMIAMLSPGFNSCEYTLNTLRYADRVKELSPQNAET  XlKCM1
XlKCM2    DEHRICVCVRKRPLNKKETTIKDLDVITIPIKDVVMVHEPKQKVD-LTRFLENQTFRFDYAFDETAPNETVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFQMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKT-KLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIEIGNSCRTSGQTSANAHSSRSHAVFQIILRKK--GKMHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELDPSRCRR  XlKCM2