The Kinesin-8 Family

The Kinesin-8 (formerly Kip3) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-8 proteins from humans and several model organisms. Kinesin-8 proteins from other organisms are also shown in the table and sequence alignment below.

Species/protein Molecular mass (kDa) Motor polarity & velocity Subcellular localization Comments
S. cerevisiae KIP3 91 ND ND
S. pombe BC2F12.13 99 ND ND
S. pombe BC649.01c 88 ND ND
D. melanogaster Klp67A   ND ND
P. falciparum MAL3P7   ND ND 

          1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360      
          '        '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '       
ScKIP3    RNPLNKVSDQVLNSMRARATKATASSINNSNATNKFSSQRR-------------------RHGGEIKFVFDKLFDETSSQARVYKETTSPLLDSVLDGFNSTVFAYGATGCGKTYTVSGTPSQPGIIFLAMEELFNKITDLKDEKDFEISLSYLEIYNERIRDLLKPET----PSKRLVIREDTQNHIKVANLSYHHPNTVEDVMDLVVQGNINRTTSPTEANEVSSRSHAVLQIHIMQTN-------KLVDLTSQHTFATLSIIDLAGSERAAATRNRGIRLHEGANINRSLLALGNCINALCLNDGSRSCHIPYRDSKLTRLLKFSLGGNCKTVMIVCISPSSSHYDETLNTLKYANRAKEIKT--KIIR  ScKIP3
SpoBC2f12 VLVFDPPDETTATLSATNRRLSTSQQSLARLSRKSNNSAGF---------------------GRDLRYAFDRVFDETATQQQVYERTARPLLDNILDGFNATIFAYGATGCGKTHTISGTMQDPGLIYLTLKELFERMDHLRDEKIFDLRLSYLEIYNETIRDLLVSPT--PNQAKPLNLREDADRRITVPGLTSLSPESLEEIIDIIMKGNANRTMSPTEANAASSRSHAVLQVTLIQKP-------RTAGINEDHTLATLSIIDLAGSERATATKLRGSRLFEGANINKSLLALGNCINALCDPHRR--AHVPYRDSKLTRLLKFSLGGNCRTVMIVCVSPSSVHYEETHNTLKYANRAKNIKTE-VLRN  SpoBC2f12
SpoBC649  IRKIVRVLDDNVLIFDPPEENPLAKVQKSLLPAGKRF--------------------------RDVRYAFDRLFGEEASQEDVYKGTTEPLLDSVLQGYNATVFAYGATGCGKTHTISGRPDDPGIIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPKSLNICEDAEQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVSSRSHAVLQIYITQTPKSGEKQEESESQNSHKVRSVFSFIDLAGSERASATKNRGKRLVEGANINRSLLALGNCINSLCEPRRR--QHVPYRDSKLTRLLKFSLGGNCRTCMIVCISPSSEHYDETHNTLKYGNRAKNIKTK-VSRN  SpoBC649
DmKlp67A  QHTNIKVAVRVRPYNVRELEQKQRSIIKVMDRSALLFDPDEEDDEFFFQGAKQPYRDITKRMNKKLTMEFDRVFDIDNSNQDLFEECTAPLVDAVLNGYNCSVFVYGATGAGKTFTMLGSEAHPGLTYLTMQDLFDKIQAQSDVRKFDVGVSYLEVYNEHVMNLLTKSG-------PLKLREDNNGVV-VSGLCLTPIYSAEELLRMLMLGNSHRTQHPTDANAESSRSHAIFQVHIRITE----------RKTDTKRTVKLSMIDLAGSERAASTKGIGVRFKEGASINKSLLALGNCINKLADGL----KHIPYRDSNLTRILKDSLGGNCRTLMVANVSMSSLTYEDTYNTLKYASRAKKIRTT-LKQN  DmKlp67A
PfMAL3P7  AYSNVKVAVRIKPIGESEENIVSIFNKNYVLIEKENEKEC----------------YLLSQKKKQSTYVFDSVFDVNATQEEVFFQTAKPLIPHVFKGINCTVFAYGATGSGKTYTMLDDKNQNGIVQLSLLELFTIINEKKCRN-IKVLMSFLEVYNETIRDLLGKEK------NKTLEVQEDVAEVKVSNLCEIEVNNYEQAMLLINEGVKNRKMSPTRANKVSSRSHAILQIYVYNE-------ILDDNMNTISYKAKLCFVDLAGSERASATSNKGERFKEGSYINQSLLALANCINSLASNRNISKVRVKYRDSKLTHLLKNSLEGNCLVVMIANINPSRTSFQESNNTLKYAFRARNIKLCATVQT  PfMAL3P7

Go to the phylogenetic tree of kinesin motor domain sequences.

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Created 15 January 1999 20:30 GMT
Modified 25 January 2005 7:26 GMT