Motor in HEM100	MTs in 100 mM PIPES	HEM + NT + taxol*	HEM100**
0.5 µM = __ µL	2 µM = __ µL	15 µL	__ µL
1 µM = __ µL	2 µM = __ µL	15 µL	__ µL
2 µM = __ µL	2 µM = __ µL	15 µL	__ µL
4 µM = __ µL	2 µM = __ µL	15 µL	__ µL
8 µM = __ µL	2 µM = __ µL	15 µL	__ µL
10 µM = __ µL	2 µM = __ µL	15 µL	__ µL
10 µM = __ µL	--	15 µL	__ µL

^*Add nucleotide and taxol to give final concentration of 2 mM and 50 µM, respectively, in assays.
^**Total volume of motor in HEM100 + MTs in 100 mM PIPES + added HEM100 = 15 µL.
Final NaCl + PIPES concentration = 50 mM
Final volume of assays = 30 µL

The assays include a control of motor without microtubules to determine the amount of motor that pellets in the absence of microtubules. This amount should be subtracted from the pellet and total concentration of motor when analyzing the data.

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Copyright 1996-2004. All rights reserved.

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Created 1 December 1999 20:15 GMT
Modified 31 October 2004 20:40 GMT