The Kinesin-6 Family

The Kinesin-6 (formerly MKLP1) family tree was found using the heuristic search method of PAUP v4.0b10 (Swofford, 2002), a maximum parsimony program, and random stepwise addition. The tree is one of two optimal trees that resulted from 600 trees that were built. The bootstrap value adjacent to the node is the percentage of 1,810 heuristic bootstrap trials in which the indicated group was found. The tree contains Kinesin-6 proteins from humans and several model organisms. Kinesin-6 proteins from other organisms are also shown in the table and sequence alignment below.

Species/protein

Molecular mass (kDa)

Motor polarity & velocity

Subcellular localization

Comments

D. melanogaster PAV-KLP 101 ND    
H.sapiens MKLP1 110 Plus, 4 µm/min Spindle, poles and midbody Separates poles at anaphase B?
C. elegans Zen4 (M03D4.1) ND ND ND  
C. griseus CHO1 109 ND Spindle, midbody Separates poles at anaphase B?
D. rerio ZfMKLP1 ND ND ND  

	  1       10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430
	  '         '        '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '         '
DmPAV     ARDPVNVFCRVRPLQSDADLTSLRVKNSTTIALNPQDQLLQHHKPHNGAQREVQYIFKHVFQPDATQQDVYAAVAQPLVENLLKGRNSLLFTYGVTGSGKTYTMTGNL--RHRGIMPRCLDVLFRTISDYQAKKFVFKPDKLNGFEILSEEDALLERQHEMNQRFAGSGRFAFRHKDSDPEIASQASVEPIPLLGLDEDNMYSVFVTYIEIYNNSVYDLLEDSGIQ----KTLQSKIIREDANRHMFVHGVTEVEVKTVEDALEVFQMGQKRKRMGHTVLNAESSRSHSVFNIRLVQAPTD--SQGENVVQDRQNITVSQLSLVDLAGSERSSRTKNTGVRLREAGNINNSLMTLRTCLEYLRENQLAASNGLAPKKVPYRDSKITHMFKNYFDGEGQVSMIVCINPRIEDYDENMQVMKFAEMTQEVQIARATP  DmPAV
HsMKLP1   LKTQLG-YCRVR-LGFPDQECCIEVINNTTVQLHTPEGYRL---NRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMP--NPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPINPNLHNLNCFVKIKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLD--ADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQ----MYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVD  HsMKLP1
CeM03D4.1 EETDSIEVVCRLCPYTGSTPSLIAIDEGSIQTVLPPAQFRR----ENAPQVEKVFRFGRVFSENDGQATVFERTSVDLILNLLKGQNSLLFTYGVTGSGKTYTMTGKPTETGTGLLPRTLDVIFNSINNRVEK-CIFYPSALNTFEIRATLDAHLKRHQMAADR------------LSTSREITDRYCEAIKLSGYNDDMVCSVFVTYVEIYNNYCYDLLEDAR-----NGVLTKREIRHDRQQQMYVDGAKDVEVSSSEEALEVFCLGEERRRVSSTLLNKDSSRSHSVFTIKLVMAPRAYETKSVYPTMDSSQIIVSQLCLVDLAGSERAKRTQNVGERLAEANSINQSLMTLRQCIEVLRRNQ--KSSSQNLEQVPYRQSKLTHLFKNYLEGNGKIRMVICVNPKPDDYDENMSALAFAEESQTIEVKKQVE  CeM03D4.1
CgCHO1    LKDPVGVYCRVRPLSCPDQECCVEVVSNTTVQLHTPEGYRL---NRNGDYKETQYSFKQVFGTHTTQKELFEVVASPLVDDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GSGGLLPRCLNMIFNSIGSFQAKRYVFKSNDRNSMEIQCEVDALLERQKREAVS--IPKTPSSKRQADPEFSDMINVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVQFDPIKPKLPQSKILREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFSIKLVQAPLD--ADGDNVLQEKEQITISQLSLVDLAGSERTNRTKAEGNRLREAGNINQSLMTLRTCMEVLRENQ----TYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEESLQVMRFAEVTQEVEVARPVD  CgCHO1
ZfMKLP1   QKDPVGVYCRVRPLGAEDEECCIEVISNTTIQLHAPDGLKA---NRNGEFKETQYSFKKVFGIKTTQRELFEDVAKPLAQDLIHGKNGLLFTYGVTGSGKTHTMTGSP--GQGGLLPRSLDMIFNSIGPYQAKRYVFKPDDKNGMEVQNQVDALLDRQKRDSQT--SVPKTPNTRRVDPEFADMISPEEACKAEGVDEDSSYSVFVSYIEIYNNYIYDLLEETPFDPIKPKPPQSKILREDQNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTQLNRESSRSHSVFIVKLAQAPLD--ADGDNVLQDKNQVNVSQLCLVDLAGSERTSRTRAEGNRLREAGNINQSLMTLRTCIEVLRENQ----MCGTNKMVPYRDSKVTHLFKNYFDGEGKVRMVVCVNPKADDYEETLLVMRFAEMTQEVEVARPVD  ZfMKLP1

Go to the phylogenetic tree of kinesin and motor domain sequences.

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Copyright 1996-2005. All rights reserved.

Created 16 July 1996 00:00 GMT
Modified 25 January 2005 7:24 GMT