Motor in HEM100	50 µM MTs	HEM100*	Tris-MgCl2- DTT	10 mM Mg·ATP	30 mM PEP	6 mM NADH	PK/LDH
--	--	19.5 µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
1 µM = __ µL	0.5 µM = 2 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
1 µM = __ µL	1.0 µM = 4 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
1 µM = __ µL	1.5 µM = 6 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
1 µM = __ µL	2.0 µM = 8 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
1 µM = __ µL	2.5 µM = 10 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
--	0.5 µM = 2 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
--	1.0 µM = 4 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
--	1.5 µM = 6 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
--	2.0 µM = 8 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL
--	2.5 µM = 10 µL	___ µL	130 µL	20 µL	20 µL	7 µL	3.5 µL

^*Total volume of Motor + MTs + HEM100 = 19.5 µL
Total [NaCl + PIPES] = 9.75 mM

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Created 6 November 1999 19:26 GMT
Modified 4 February 2000 20:50 GMT